ArtNr |
ABC-ASC10927 |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Lieferbar |
|
Primary Accession |
Q5T1C6 |
Application |
WB, IHC-P, IF, E |
Bio Background |
THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. |
Bio References |
Maira SM, Galetic I, Brazil DP, et al. Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane. Science2001; 294:374-80.Knobbe CB, Reifberger J, Blaschke B, et al. Hypermethylation and transcriptional downregulation of the carboxyl-terminal modulator protein gene in glioblastomas. J. Natl. Cancer Inst.2004; 96:483-6.Zhao H, Martin BM, Bisoffi M, et al. The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the hotdog-fold family. Biochemistry2009; 48:5507-9.Parcellier A, Tintignac LA, Zhuravleva E, et al. Carboxy-terminal modulator protein (CTMP) is a mitochondrial protein that sensitizes cells to apoptosis. Cell Signal.2009; 21:639-50. |
Clonality |
Polyclonal |
Gene ID |
117145 |
Gene Name |
THEM4 |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 µg/mL. |
Type image 1 |
WB |
Legend image 2 |
Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 µg/mL. |
Type image 2 |
IHC |
Legend image 3 |
Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 µg/mL. |
Type image 3 |
IF |
Other Accession |
NP_444283; 76159293 |
Isotype |
IgG |
Calculated MW |
27130 |
Antigen Source |
Homo sapiens |
Target/Specificity |
THEM4; The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4. |
Purification |
THEM4 Antibody is affinity chromatography purified via peptide column. |
Reconstitution & Storage |
THEM4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Positive Control |
Human Liver Tissue Lysate; |
Buffer |
THEM4 Antibody is supplied in PBS containing 0.02% sodium azide. |
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