ArtNr |
ABC-ASC10880 |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, ICC, ELISA |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Lieferbar |
|
Primary Accession |
Q96IU2 |
Application |
WB, ICC, E |
Bio Background |
ZBED3 Antibody: Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression. |
Bio References |
Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem.2009; 284:6683-9. |
Clonality |
Polyclonal |
Gene ID |
84327 |
Gene Name |
ZBED3 |
Reactivity |
H |
Legend image 1 |
Western blot analysis of ZBED3 in Jurkat cell lysate with ZBED3 antibody at 1 µg/mL. |
Type image 1 |
WB |
Legend image 2 |
Immunocytochemistry of ZBED3 in A549 cells with ZBED3 antibody at 20 µg/mL. |
Type image 2 |
ICC |
Other Accession |
NP_115743; 14150185 |
Isotype |
IgG |
Calculated MW |
25132 |
Antigen Source |
Homo sapiens |
Target/Specificity |
ZBED3; This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2. |
Purification |
ZBED3 Antibody is affinity chromatography purified via peptide column. |
Reconstitution & Storage |
ZBED3 antibody can be stored at 4? for three months and -20?, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 µg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. |
Positive Control |
Jurkat Cell Lysate; |
Buffer |
ZBED3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.