JPH4 Antibody

Q96JJ6

JPH4 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain. In the CNS, both JPH3 and JPH4 are expressed throughout neural sites and contribute to the subsurface cistern formation in neurons. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions.

Polyclonal

JPH4

Western blot analysis of JPH4 in 293 cell lysate with JPH4 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of JPH4 in human brain tissue with JPH4 antibody at 2.5 µg/mL.

Immunofluorescence of JPH4 in Human Brain cells with JPH4 antibody at 20 µg/mL.

AAH55429; 33585440

65861

JPH4;

JPH4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

JPH4 antibody can be used for detection of JPH4 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.

JPH4 Antibody is supplied in PBS containing 0.02% sodium azide.