ArtNr |
ABC-ASC10191 |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, IF, ICC, ELISA |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
RNF85, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, MGC:3310, TRAF6 Antibody: RNF85 |
Lieferbar |
|
Primary Accession |
Q9Y4K3 |
Application |
WB, IF, ICC, E |
Bio Background |
TRAF6 Antibody: Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK. |
Bio References |
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79.Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72.Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63. |
Clonality |
Polyclonal |
Gene ID |
7189 |
Gene Name |
TRAF6 |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 µg/mL in the presence (A) or absence (B) of 1 µg blocking peptide. |
Type image 1 |
WB |
Legend image 2 |
Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 µg/mL. |
Type image 2 |
ICC |
Legend image 3 |
Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 µg/mL. |
Type image 3 |
IF |
Other Accession |
NP_004611; 4759254 |
Isotype |
IgG |
Calculated MW |
59573 |
Antigen Source |
Homo sapiens |
Target/Specificity |
TRAF6; |
Purification |
TRAF6 Antibody is Ion exchange chromatography purified. |
Reconstitution & Storage |
TRAF6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
Positive Control |
PC-3 Cell Lysate; K562 Cell Lysate |
Buffer |
TRAF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.