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LCAT Antibody (Center)

LCAT Antibody (Center)

ArtNr	ABC-AP9868C-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	LCAT, Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Phospholipid-cholesterol acyltransferase
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Primary Accession	P04180
Antigen Type	Synthetic Peptide
Application	FC, IF, IHC-P, WB
Bio Background	LCAT encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport.
Bio References	Weissglas-Volkov, D., et al. Circ Cardiovasc Genet 3(1):31-38(2010) McGeachie, M., et al. Circulation 120(24):2448-2454(2009) Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009) : Voora, D., et al. Circ Cardiovasc Genet 1(2):100-106(2008)
Clonality	Polyclonal
Clone/Animal Names	RB23365
Gene ID	3931
Gene Name	LCAT
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of LCAT Antibody (Center) (Cat. #AP9868c) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of LCAT Antibody (Center) (Cat. #AP9868c) in K562 cell line lysates (35ug/lane). LCAT (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	LCAT Antibody (Center) (Cat. #AP9868c) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LCAT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	LCAT Antibody (Center) (Cat. #AP9868c) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Isotype	Rabbit Ig
Calculated MW	49578
Antigen Region	285-313
Antigen Source	HUMAN
Target/Specificity	This LCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human LCAT.
NCBI Accession	NP_000220.1

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ABC-AP9868C-ev-datasheet.pdf

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Menge: 80 ul

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