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LDHA Antibody (Center)

LDHA Antibody (Center)

ArtNr	ABC-AP9523c-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	LDHA, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-A, LDH-M
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Primary Accession	P00338
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria.
Bio References	Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010) Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010) Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009) Xie, H., et al. Mol. Cancer Ther. 8(3):626-635(2009) Koukourakis, M.I., et al. Oncology 77(5):285-292(2009) Rikova, K., et al. Cell 131(6):1190-1203(2007) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Clonality	Polyclonal
Clone/Animal Names	RB24077
Gene ID	3939
Gene Name	LDHA
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	LDHA Antibody (Center) (Cat. #AP9523c) western blot analysis in Ramos, A375 cell line lysates (35ug/lane).This demonstrates the LDHA antibody detected the LDHA protein (arrow).
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human skin tissue reacted with LDHA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	LDHA Antibody (Center) (Cat. #AP9523c) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	P04642; P00339; Q9BE24; P19858
Predicted	B, Pr, Pig, Rat
Isotype	Rabbit Ig
Calculated MW	36689
Antigen Region	154-182
Antigen Source	HUMAN
Target/Specificity	This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the Central region of human LDHA.
NCBI Accession	NP_001128711.1; NP_001158886.1; NP_001158887.1; NP_001158888.1; NP_005557.1

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