ENTPD2 Antibody (N-term)

Synthetic Peptide

ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.

Polyclonal

954

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-ENTPD2 Antibody at 1:1000 dilution
Lane 1: human heart lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: human brain lysate
Lane 5: human skeletal muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Western blot analysis of ENTPD2 Antibody (N-term) (Cat. #AP9325a) in K562 cell line lysates (35ug/lane). ENTPD2 (arrow) was detected using the purified Pab.

1:1000

IHC-P

Overlay histogram showing K562 cells stained with AP9325a(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9325a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

53665

HUMAN

NP_001237.1; NP_982293.1