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GPX1 Antibody (C-term)

GPX1 Antibody (C-term)

ArtNr	ABC-AP9315b-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GPX1, Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase
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Primary Accession	P07203
Antigen Type	Synthetic Peptide
Application	WB, IF, IHC-P, FC
Bio Background	GPX1 encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.
Bio References	Moyer, A.M., et.al., Cancer Epidemiol. Biomarkers Prev. 19 (3), 811-821 (2010) Akimoto, A.K., et.al., Free Radic. Res. 44 (3), 322-331 (2010) Cao, C., et.al., J. Biol. Chem. 278 (41), 39609-39614 (2003)
Clonality	Polyclonal
Clone/Animal Names	RB22987
Gene ID	2876
Gene Name	GPX1 (HGNC:4553)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	Confocal immunofluorescent analysis of GPX1 Antibody (C-term)(Cat#AP9315b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T/17 whole cell lysate Lane 4: human kidney lysate Lane 5: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	Western blot analysis of lysates from THP-1 cell line ，mouse liver and rat liver tissue (from left to right), using GPX1 Antibody (C-term)(Cat. #AP9315b).AP9315b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GPX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 5	IHC-P
Dilution image 5	1:10~50
Legend image 6	Overlay histogram showing HepG2 cells stained with AP9315b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9315b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 6	FC
Dilution image 6	1:25
Legend image 7	Overlay histogram showing HepG2 cells stained with AP9315b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9315b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 7	FC
Dilution image 7	1:25
Isotype	Rabbit Ig
Calculated MW	22088
Antigen Region	164-193
Antigen Source	HUMAN
Target/Specificity	This GPX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1.
NCBI Accession	NP_000572.2; NP_958799.1

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Menge: 80 ul

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