ArtNr |
ABC-AP9099c-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, FC |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
COL17A1, BP180, BPAG2, LAD-1, Collagen alpha-1(XVII) chain, LABD97, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, Linear IgA disease antigen 1, 120 kDa linear IgA disease antigen, 120 kDa linear IgA dermatosis antigen, 97 kDa linear IgA disease antigen, 97 kDa linear IgA bullous dermatosis antigen, 97 kDa LAD antigen, 97-LAD, Linear IgA bullous disease antigen of 97 kDa |
Lieferbar |
|
Primary Accession |
Q9UMD9 |
Antigen Type |
Synthetic Peptide |
Application |
WB, FC |
Bio Background |
COL17A1 encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. |
Bio References |
Di Zenzo, G., et.al., J. Invest. Dermatol. 130 (4), 1040-1047 (2010)Ujiie, H., et.al., J. Immunol. 184 (4), 2166-2174 (2010) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB22700 |
Gene ID |
1308 |
Gene Name |
COL17A1 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-COL17A1 Antibody (Center) at 1:2000 dilutionLane 1: A2058 whole cell lysateLane 2: A375 whole cell lysateLane 3: HL-60 whole cell lysateLane 4: MCF-7 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 150 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
Overlay histogram showing A431 cells stained with AP9099c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9099c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 2 |
FC |
Dilution image 2 |
1:25 |
Isotype |
Rabbit Ig |
Calculated MW |
150419 |
Antigen Region |
475-504 |
Antigen Source |
HUMAN |
Target/Specificity |
This COL17A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human COL17A1. |
NCBI Accession |
NP_000485.3 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.