Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, FC, IHC-P |
Menge |
80 ul |
Host |
Rabbit |
ArtNr |
ABC-AP8775a-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
Q9UKI2 |
Antigen Type |
Synthetic Peptide |
Application |
FC, WB, IHC-P |
Bio Background |
CDC42EP3 is probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. It induces pseudopodia formation in fibroblasts. |
Bio References |
Simpson, J.C., et.al., EMBO Rep. 1 (3), 287-292 (2000) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB23512 |
Gene ID |
10602 |
Gene Name |
CDC42EP3 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of CDC42EP3 Antibody (N-term) (Cat. #AP8775a) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term) (Cat. #AP8775a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Legend image 4 |
CDC42EP3 Antibody (N-term) (Cat. #AP8775a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Other Accession |
Q9CQC5; NP_006440 |
Predicted |
M |
Isotype |
Rabbit Ig |
Calculated MW |
27678 |
Antigen Region |
1-30 |
Antigen Source |
HUMAN |
Target/Specificity |
This CDC42EP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CDC42EP3. |
NCBI Accession |
NP_001257365.1; NP_001257366.1; NP_001257367.1; NP_006440.2 |
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