Vergleich

LTF Antibody

ArtNr ABC-AP8698A-ev
Hersteller Abcepta
Menge 80 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen WB, FC
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products Lactotransferrin, LTF, Lactoferrin, LF, Talalactoferrin, Kaliocin-1, Lactoferroxin-A, Lactoferroxin-B, Lactoferroxin-C, GIG12, Growth-inhibiting protein 12, Lfcin-H, 3421-, Lactoferricin-H
Lieferbar
Primary Accession
P02788
Antigen Type
Recombinant Protein
Application
FC, WB
Bio Background
LTF is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis.
Bio References
Willnow, T.E., et.al., J. Biol. Chem. 267 (36), 26172-26180 (1992)
Clonality
Polyclonal
Clone/Animal Names
RB22673
Gene ID
4057
Gene Name
LTF
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
Western blot analysis of LTF Antibody (Cat. #AP8698a) in MDA-MB231 cell line lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Western blot analysis of LTF Antibody (Cat. #AP8698a) in mouse spleen tissue lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
All lanes : Anti-LTF Antibody at 1:4000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Human plasma tissue lysate
Lane 3: Human fetal kidney tissue lysate
Lane 4: Human breast tissue lysate
Lane 5: Human uterus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 78 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3
WB
Dilution image 3
1:4000
Legend image 4
All lanes : Anti-LTF Antibody at 1:1000-2000 dilution
Lane 1: Human plasma tissue lysate
Lane 2: Hela whole cell lysate
Lane 3: Human uterus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 78 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4
WB
Dilution image 4
1:1000-2000
Legend image 5
All lanes : Anti-LTF Antibody at 1:1000 dilution
Lane 1: Human plasma tissue lysate
Lane 2: Human fetal kidney tissue lysate
Lane 3: Human uterus tissue lysate
Lane 4: Human skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 78 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 5
WB
Dilution image 5
1:1000
Legend image 6
LTF Antibody (Cat. #AP8698a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 6
FC
Dilution image 6
1:10~50
Isotype
Rabbit Ig
Calculated MW
78182
Antigen Source
HUMAN
Target/Specificity
This LTF antibody is generated from rabbits immunized with LTF recombinant protein.
NCBI Accession
NP_001186078.1; NP_002334.2

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 80 ul
Lieferbar: In stock
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