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PCK2 Antibody (N-term)

PCK2 Antibody (N-term)

ArtNr	ABC-AP8094a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	mitochondrial, PCK2, PEPCK2, Phosphoenolpyruvate carboxykinase [GTP], PEPCK-M
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Primary Accession	Q16822
Antigen Type	Synthetic Peptide
Application	IF, WB, IHC-P
Bio Background	This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
Bio References	Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Modaressi, S., et al., Biochem. J. 333 (Pt 2), 359-366 (1998). Modaressi, S., et al., Biochem. J. 315 (Pt 3), 807-814 (1996).
Clonality	Polyclonal
Clone/Animal Names	RB3640
Gene ID	5106
Gene Name	PCK2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of PCK2 Antibody (N-term)(Cat#AP8094a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of anti-PCK2 Antibody (N-term) (Cat.#AP8094a) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) (Cat. #AP8094a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 4	IHC-P
Dilution image 4	1:50~100
Isotype	Rabbit Ig
Calculated MW	70699
Antigen Region	24-54
Antigen Source	HUMAN
Target/Specificity	This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2.
NCBI Accession	NP_001018083.1; NP_004554.2

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Menge: 400 ul

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