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PAK5 Antibody

PAK5 Antibody

ArtNr	ABC-AP7930a-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PAK5, PAK7, PAK-5, Serine/threonine-protein kinase PAK 7, p21-activated kinase 5, p21-activated kinase 7, KIAA1264, PAK-7
Lieferbar
Primary Accession	Q9P286
Antigen Type	Synthetic Peptide
Application	IHC-P, WB, IHC-P-Leica
Bio Background	PAK5 is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression.
Bio References	Jaffer, Z.M., et al., Int. J. Biochem. Cell Biol. 34(7):713-717 (2002). Pandey, A., et al., Oncogene 21(24):3939-3948 (2002). Dan, C., et al., Mol. Cell. Biol. 22(2):567-577 (2002). Cau, J., et al., J. Cell Biol. 155(6):1029-1042 (2001).
Clonality	Polyclonal
Clone/Animal Names	RB1235
Gene ID	57144
Gene Name	PAK5 (HGNC:15916)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-PAK5 Antibody at 1:1000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of anti-PAK5 Pab (Cat. #AP7930a) in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK5 (arrow) was detected using purified Pab. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Anti-PAK5 Antibody at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	Immunohistochemical analysis of AP7930A on paraffin-embedded Human cerebellum tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:100
Isotype	Rabbit Ig
Calculated MW	80745
Antigen Region	168-198
Antigen Source	HUMAN
Target/Specificity	This PAK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from human PAK5.
NCBI Accession	NP_065074.1; NP_817127.1

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Menge: 80 ul

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