ArtNr |
ABC-AP7622a |
Hersteller |
Abcepta
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
EPHB1, NET, ELK, EPHT2, HEK6, Ephrin type-B receptor 1, EPH tyrosine kinase 2, EPH-like kinase 6, Neuronally-expressed EPH-related tyrosine kinase, Tyrosine-protein kinase receptor EPH-2, EK6, hEK6 |
Lieferbar |
|
Primary Accession |
P54762 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
Bio References |
Prevost, N., et al., Proc. Natl. Acad. Sci. U.S.A. 99(14):9219-9224 (2002).Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000).Holder, N., et al., Development 126(10):2033-2044 (1999).Stein, E., et al., J. Biol. Chem. 273(3):1303-1308 (1998). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB1648 |
Gene ID |
2047 |
Gene Name |
EPHB1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Western blot analysis of lysates from A431, Hela, MDA-MB-453 cell line (from left to right), using EPHB1 Antibody (H970)(Cat. #AP7622a). AP7622a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of anti-EphB1 Pab (Cat. #AP7622a) in mouse brain tissue. EphB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
EPHB1 Antibody (H970) (Cat. #AP7622a) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the EPHB1 antibody detected the EPHB1 protein (arrow). |
Type image 3 |
WB |
Dilution image 3 |
1:1000 |
Legend image 4 |
Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d Loss of EphB1 expression in gastric cancer cells.(Provided by Jian-dong Wang, Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine) |
Type image 4 |
IHC-P |
Dilution image 4 |
1:50~100 |
Legend image 5 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Type image 5 |
IHC-P |
Dilution image 5 |
1:50~100 |
Isotype |
Rabbit Ig |
Calculated MW |
109885 |
Antigen Region |
955-984 |
Antigen Source |
HUMAN |
Target/Specificity |
This EphB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-984 amino acids from the C-terminal region of human EphB1. |
NCBI Accession |
NP_004432.1 |
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