NCF4 Antibody (C-term)

Synthetic Peptide

NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2(NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity.

Polyclonal

4689

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of NCF4 antibody (C-term)(Cat.#AP7615b) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.

1:1000

IHC-P

NCF4 Antibody (C-term) (Cat. #AP7615b) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

39032

HUMAN

NP_000622.2; NP_038202.2