Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, IHC-P |
Menge |
80 ul |
Host |
Rabbit |
ArtNr |
ABC-AP7000E-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
Q9UQB9 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase. |
Bio References |
Kimura, M., et al., J. Biol. Chem. 274(11):7334-7340 (1999).Tseng, T.C., et al., DNA Cell Biol. 17(10):823-833 (1998).Bernard, M., et al., Genomics 53(3):406-409 (1998). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB6505 |
Gene ID |
6795 |
Gene Name |
AURKC |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
The anti-Aurora C Pab (Cat. #AP7000e) is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Formalin-fixed and paraffin-embedded H.testis tissue reacted with Aurora-C Antibody (N-term M1) (Cat#AP7000e). |
Type image 2 |
IHC-P |
Dilution image 2 |
1:100 |
Isotype |
Rabbit Ig |
Calculated MW |
35591 |
Antigen Region |
1-30 |
Antigen Source |
HUMAN |
Target/Specificity |
This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Aurora-C. |
NCBI Accession |
NP_001015878.1; NP_001015879.1; NP_003151.2 |
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