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Calponin-1 Antibody (N-term)

Calponin-1 Antibody (N-term)

ArtNr	ABC-AP6795A-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CNN1, smooth muscle, Calponin-1, Basic calponin, Calponin H1
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Primary Accession	P51911
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P
Bio Background	Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Bio References	Marsh, W.L., et.al., Appl. Immunohistochem. Mol. Morphol. 17 (3), 216-219 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB19472
Gene ID	1264
Gene Name	CNN1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of Calponin-1 Antibody (N-term) (Cat. #AP6795a) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of lysates from Hela, MDA-MB-453 cell line (from left to right), using Calponin-1 Antibody (N-term)(Cat. #AP6795a). AP6795a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	Q08290; Q08091; Q2HJ38; Q7YRL2
Predicted	B, M, Rat, Sh
Isotype	Rabbit Ig
Calculated MW	33170
Antigen Region	10-39
Antigen Source	HUMAN
Target/Specificity	This Calponin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human Calponin-1.
NCBI Accession	NP_001290.2

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