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Smad7 Antibody (Center)

Smad7 Antibody (Center)

ArtNr	ABC-AP6753C-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Smad7, SMAD7, MADH7, MADH8, Mothers against decapentaplegic homolog 7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, hSMAD7
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Primary Accession	O15105
Antigen Type	Synthetic Peptide
Application	IF, IHC-P, WB
Bio Background	Smad7 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta super family.
Bio References	Hirata, H., et.al., Cancer 115 (19), 4488-4503 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB18598
Gene ID	4092
Gene Name	SMAD7
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	"Fluorescent confocal image of A431 cell stained with Smad7 Antibody (Center)(Cat#AP6753c). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).Smad7 immunoreactivity is localized to cytoplasm significantly. "
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of Smad7 Antibody (Center) (Cat. #AP6753c) in ZR-75-1 cell line lysates (35ug/lane). Smad7 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Smad7 Antibody (Center) (Cat. #AP6753c) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Smad7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Other Accession	O88406
Predicted	Rat
Isotype	Rabbit Ig
Calculated MW	46426
Antigen Region	195-224
Antigen Source	HUMAN
Target/Specificity	This Smad7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human Smad7.
NCBI Accession	NP_001177750.1; NP_001177751.1; NP_001177752.1; NP_005895.1

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ABC-AP6753C-ev-datasheet.pdf

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Menge: 80 ul

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Lieferung vsl. bis 08.11.2024

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