ArtNr |
ABC-AP6662b-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, FC, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
PDIA6, P5, ERP5, TXNDC7, Protein disulfide-isomerase A6, ERp5, Endoplasmic reticulum protein 5, ER protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7 |
Lieferbar |
|
Primary Accession |
Q15084 |
Antigen Type |
Synthetic Peptide |
Application |
IF, IHC-P, WB, FC |
Bio Background |
Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins. |
Bio References |
Hayano, T., Gene 164 (2), 377-378 (1995) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB20199 |
Gene ID |
10130 |
Gene Name |
PDIA6 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M, Rat |
Legend image 1 |
Fluorescent image of HepG2 cells stained with XAF1 PDIA6 Antibody (Center K159)(Cat#AP6662b). AP6662b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). |
Type image 1 |
IF |
Dilution image 1 |
1:100 |
Legend image 2 |
Western blot analysis of PDIA6 antibody (Center K159) (Cat.# AP6662b) in Y79 cell line lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Western blot analysis of PDIA6 antibody (Center K159) (Cat.# AP6662b) in mouse stomach tissue lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab. |
Type image 3 |
WB |
Dilution image 3 |
1:1000 |
Legend image 4 |
Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using PDIA6 Antibody (Center K159)(Cat. #AP6662b). AP6662b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
Type image 4 |
WB |
Dilution image 4 |
1:1000 |
Legend image 5 |
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 5 |
IHC-P |
Dilution image 5 |
1:50~100 |
Legend image 6 |
Immunohistochemical analysis of paraffin-embedded H. liver section using PDIA6 Antibody (Center K159)(Cat#AP6662b). AP6662b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. |
Type image 6 |
IHC-P |
Dilution image 6 |
1:100 |
Legend image 7 |
Immunohistochemical analysis of paraffin-embedded H. brain section using PDIA6 Antibody (Center K159)(Cat#AP6662b). AP6662b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. |
Type image 7 |
IHC-P |
Dilution image 7 |
1:100 |
Isotype |
Rabbit Ig |
Calculated MW |
48121 |
Antigen Region |
144-172 |
Antigen Source |
HUMAN |
Target/Specificity |
This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6. |
NCBI Accession |
NP_005733.1 |
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