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HPX Antibody (Center)

HPX Antibody (Center)

ArtNr	ABC-AP6653c-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HPX, Hemopexin, Beta-1B-glycoprotein
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Primary Accession	P02790
Antigen Type	Synthetic Peptide
Application	IHC-P-Leica, WB, FC
Bio Background	HPX is a plasma glycoprotein that binds heme with high affinity. The protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.
Bio References	Ascenzi, P., IUBMB Life 59 (11), 700-708 (2007)
Clonality	Polyclonal
Clone/Animal Names	RB18728
Gene ID	3263
Gene Name	HPX
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	All lanes : Anti-HPX Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Human plasma lysate Lane 3: Mouse plasma lysate Lane 4: Rat plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	All lanes : Anti-HPX Antibody (Center) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Immunohistochemical analysis of paraffin-embedded Human liver tissue using AP6653c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P-Leica
Dilution image 3	1:500
Legend image 4	Immunohistochemical analysis of paraffin-embedded Human liver tissue using AP6653c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 4	IHC-P-Leica
Dilution image 4	1:500
Legend image 5	HPX Antibody (Center) (Cat. #AP6653c) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Isotype	Rabbit Ig
Calculated MW	51676
Antigen Region	200-227
Antigen Source	HUMAN
Target/Specificity	This HPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the Central region of human HPX.
NCBI Accession	NP_000604.1

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