ArtNr |
ABC-AP6625A-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, FC, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
KLF11, FKLF, TIEG2, Krueppel-like factor 11, TGFB-inducible early growth response protein 2, TIEG-2, Transforming growth factor-beta-inducible early growth response protein 2 |
Lieferbar |
|
Primary Accession |
O14901 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, FC, IF, WB |
Bio Background |
TIEG2 is a transcription factor. The protein activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. It represses transcription of SMAD7 which enhances TGF-beta signaling. It induces apoptosis. |
Bio References |
Kuroda, E., Endocr. J. 56 (2), 275-286 (2009)Ma, L., J. Clin. Endocrinol. Metab. 93 (9), 3644-3649 (2008) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB19899 |
Gene ID |
8462 |
Gene Name |
KLF11 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Confocal immunofluorescent analysis of TIEG2 Antibody (N-term)(Cat. #AP6625a) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Anti-TIEG2 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human brain tissue reacted with TIEG2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Legend image 4 |
Flow cytometric analysis of 293 cells using TIEG2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Other Accession |
Q8K1S5 |
Predicted |
M |
Isotype |
Rabbit Ig |
Calculated MW |
55139 |
Antigen Region |
7-36 |
Antigen Source |
HUMAN |
Target/Specificity |
This TIEG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human TIEG2. |
NCBI Accession |
NP_001171187.1; NP_001171189.1; NP_003588.1 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.