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CD14 Antibody (N-term)

CD14 Antibody (N-term)

ArtNr	ABC-AP6294A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, membrane-bound form, urinary form
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Primary Accession	P08571
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB, IF, IHC-P-Leica
Bio Background	CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Bio References	Donati, M., J. Periodontol. 79 (3), 517-524 (2008) Yuan, F.F., Immunol. Cell Biol. 86 (3), 268-270 (2008) Setoguchi, M., Biochim. Biophys. Acta 1008 (2), 213-222 (1989) Goyert, S.M., Science 239 (4839), 497-500 (1988)
Clonality	Polyclonal
Clone/Animal Names	RB14105
Gene ID	929
Gene Name	CD14
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling CD14 with AP6294A at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and membrance staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lane 3: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: THP-1 whole cell lysate Lane 2: Human lung tissue lysate Lane 3: Human liver tissue lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 5	WB
Dilution image 5	1:2000
Legend image 6	Immunohistochemical analysis of paraffin-embedded human liver tissue using AP6294A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 6	IHC-P-Leica
Dilution image 6	1:1000
Legend image 7	Overlay histogram showing Jurkat cells stained with AP6294A(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 7	FC
Dilution image 7	1:25
Isotype	Rabbit IgG
Calculated MW	40076
Antigen Region	54-83
Antigen Source	HUMAN
Target/Specificity	This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.
NCBI Accession	NP_000582.1; NP_001035110.1; NP_001167575.1; NP_001167576.1

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Menge: 400 ul

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