ArtNr |
ABC-AP52835 |
Hersteller |
Abcepta
|
Menge |
100 ul |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, ICC |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2. |
Lieferbar |
|
Primary Accession |
P39748 |
Antigen Type |
Recombinant Protein |
Application |
WB, ICC |
Bio Background |
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. |
Bio References |
Murray J.M., et al.Mol. Cell. Biol. 14:4878-4888(1994).Hiraoka L.R., et al.Genomics 25:220-225(1995).Taylor T.D., et al.Nature 440:497-500(2006).Robins P., et al.J. Biol. Chem. 269:28535-28538(1994).Shen B., et al.J. Biol. Chem. 271:9173-9176(1996). |
Clonality |
Monoclonal |
Gene ID |
2237 |
Gene Name |
FEN1 {ECO:0000255|HAMAP-Rule:MF_03140} |
Subtitle |
Purified Mouse Monoclonal Antibody (Mab) |
Reactivity |
H, M |
Legend image 1 |
Western blot detection of FEN-1 in Hela, Jurkat, 3T3, COS7, PC-12, C6, Raw264.7, Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400). |
Type image 2 |
ICC |
Dilution image 2 |
1:400 |
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