ArtNr |
ABC-AP5231a-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, FC |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
HIST1H1B, Histone H1b, H1F5, Histone H1a, Histone H1s-3, Histone H15 |
Lieferbar |
|
Primary Accession |
P16401 |
Antigen Type |
Synthetic Peptide |
Application |
FC, WB |
Bio Background |
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. |
Bio References |
Happel, N., et al. J. Mol. Biol. 386(2):339-350(2009)Trojer, P., et al. Cell 129(5):915-928(2007)Wisniewski, J.R., et al. Mol. Cell Proteomics 6(1):72-87(2007) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB30034 |
Gene ID |
3009 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Anti-HIST1H1B Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
HIST1H1B Antibody (N-term) (Cat. #AP5231a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 2 |
FC |
Dilution image 2 |
1:10~50 |
Other Accession |
P02251 |
Predicted |
Rb |
Isotype |
Rabbit Ig |
Calculated MW |
22580 |
Antigen Region |
33-60 |
Antigen Source |
HUMAN |
Target/Specificity |
This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human HIST1H1B. |
NCBI Accession |
NP_005313.1 |
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