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phospho-Sox2(S246) Antibody

phospho-Sox2(S246) Antibody

ArtNr	ABC-AP3750a-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	IF, Dot
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SOX2, Transcription factor SOX-2
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Primary Accession	P48431
Antigen Type	Synthetic Peptide
Application	DB, IF
Bio Background	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Bio References	References for protein: 1.Gen, Y., et al. Cancer Genet. Cytogenet. 202(2):82-93(2010) 2.Ji, J., et al. Hum. Pathol. 41(10):1438-1447(2010) 3.Fang, X., et al. OMICS 14(4):369-384(2010) 4.Sholl, L.M., et al. Am. J. Surg. Pathol. 34(8):1193-1198(2010) 5.Zhang, D., et al. BMC Med. Genet. 11, 116 (2010) References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
Clonality	Polyclonal
Clone/Animal Names	RB26203
Gene ID	6657
Gene Name	SOX2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3750a phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.
Type image 1	IF
Dilution image 1	1:200
Legend image 2	Dot blot analysis of anti-phospho-Sox2-pS246 Phospho-specific Pab (Cat. #AP3750a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Type image 2	DB
Dilution image 2	1:500
Other Accession	P48432; NP_003097.1; P54231
Predicted	M, Sh
Isotype	Rabbit Ig
Calculated MW	34310
Antigen Source	HUMAN
Target/Specificity	This Sox2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human Sox2.
NCBI Accession	NP_003097.1

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Menge: 80 ul

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