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Phospho-SMAD3(S208) Antibody

Phospho-SMAD3(S208) Antibody

ArtNr	ABC-AP3249a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	IF, Dot
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SMAD3, Smad3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3, Mad3, SMAD 3, Mothers against DPP homolog 3, hSMAD3, hMAD-3, MAD homolog 3
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Primary Accession	P84022
Antigen Type	Synthetic Peptide
Application	DB, IF
Bio Background	SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
Bio References	References for protein: 1.Imoto, S., et al., FEBS Lett. 579(13):2853-2862 (2005). 2.Dubrovska, A., et al., Oncogene 24(14):2289-2297 (2005). 3.Furumatsu, T., et al., J. Biol. Chem. 280(9):8343-8350 (2005). 4.Kobayashi, T., et al., Biochem. Biophys. Res. Commun. 327(2):393-398 (2005). 5.Kamaraju, A.K., et al., J. Biol. Chem. 280(2):1024-1036 (2005). References for HeLa cell line: 1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695–710. [PubMed:13052828]. 2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141–50. [PubMed: 9892199]. 3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277–84. [PubMed: 19450234]. 4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1–8. [PubMed:20143388].
Clonality	Polyclonal
Clone/Animal Names	RB31592, RB31594
Gene ID	4088
Gene Name	SMAD3
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Fluorescent confocal image of HeLa cells stained with phospho-SMAD3-S208 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3249a phospho-SMAD3-S208 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence).Note the highly specific localization of the phospho-SMAD3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000166949).
Type image 1	IF
Dilution image 1	1:200
Legend image 2	Confocal immunofluorescent analysis of Phospho-SMAD3-S208 Antibody(Cat#AP3249a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Type image 2	IF
Dilution image 2	1:10~50
Legend image 3	Dot blot analysis of anti-hSMAD3-S208 Phospho-specific Pab (Cat. #AP3249a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Type image 3	DB
Dilution image 3	1:500
Other Accession	P84025; P84024; Q8BUN5; P84023
Predicted	C, M, Pig, Rat
Isotype	Rabbit Ig
Calculated MW	48081
Antigen Source	HUMAN
Target/Specificity	This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S208 of human SMAD3.
NCBI Accession	NP_001138574.1; NP_001138575.1; NP_001138576.1; NP_005893.1

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Menge: 400 ul

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