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TPI1 Antibody (N-term)

ArtNr ABC-AP2917a
Hersteller Abcepta
Menge 400 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products TPI1, TIM, Triosephosphate isomerase, TPI, Triose-phosphate isomerase
Lieferbar
Primary Accession
P60174
Antigen Type
Synthetic Peptide
Application
FC, IHC-P, WB
Bio Background
TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
Bio References
Martins-de-Souza, D., et.al., BMC Psychiatry 9, 17 (2009)
Clonality
Polyclonal
Clone/Animal Names
RB20861
Gene ID
7167
Gene Name
TPI1
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
Western blot analysis of TPI1 Antibody (N-term) (Cat. #AP2917a) in Y79(lane 1), CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml)
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term) (Cat. #AP2917a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
All lanes : Anti-TPI1 Antibody (N-term) at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 31 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3
WB
Dilution image 3
1:1000
Legend image 4
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4
IHC-P
Dilution image 4
1:10~50
Legend image 5
TPI1 Antibody (N-term) (Cat. #AP2917a) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5
FC
Dilution image 5
1:10~50
Other Accession
P48500; P00939; Q29371; P17751; Q60HC9; Q5E956
Predicted
B, Pr, Pig, Rb, Rat
Isotype
Rabbit Ig
Calculated MW
26669
Antigen Region
68-96
Antigen Source
HUMAN
Target/Specificity
This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1.
NCBI Accession
NP_000356.1; NP_001152759.1; NP_001244955.1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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