ArtNr |
ABC-AP2917a |
Hersteller |
Abcepta
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, FC, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
TPI1, TIM, Triosephosphate isomerase, TPI, Triose-phosphate isomerase |
Lieferbar |
|
Primary Accession |
P60174 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IHC-P, WB |
Bio Background |
TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. |
Bio References |
Martins-de-Souza, D., et.al., BMC Psychiatry 9, 17 (2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB20861 |
Gene ID |
7167 |
Gene Name |
TPI1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Western blot analysis of TPI1 Antibody (N-term) (Cat. #AP2917a) in Y79(lane 1), CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml) |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term) (Cat. #AP2917a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
All lanes : Anti-TPI1 Antibody (N-term) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 3 |
WB |
Dilution image 3 |
1:1000 |
Legend image 4 |
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 4 |
IHC-P |
Dilution image 4 |
1:10~50 |
Legend image 5 |
TPI1 Antibody (N-term) (Cat. #AP2917a) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 5 |
FC |
Dilution image 5 |
1:10~50 |
Other Accession |
P48500; P00939; Q29371; P17751; Q60HC9; Q5E956 |
Predicted |
B, Pr, Pig, Rb, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
26669 |
Antigen Region |
68-96 |
Antigen Source |
HUMAN |
Target/Specificity |
This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1. |
NCBI Accession |
NP_000356.1; NP_001152759.1; NP_001244955.1 |
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