MTHFD1 Antibody (Center P550)

Synthetic Peptide

MTHFD1 is a protein that possesses three distinct enzymatic activities, 5, 10-methylenetetrahydrofolate dehydrogenase, 5, 10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

Polyclonal

4522

Purified Rabbit Polyclonal Antibody (Pab)

Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) (Cat#AP2788f) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

1:10~50

WB

All lanes : Anti-MTHFD1 Antibody (Center P550) at 1:1000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 102 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P

P27653; Q922D8; NP_005947

Rabbit Ig

535-562

This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.