ArtNr |
ABC-AP2505b |
Hersteller |
Abcepta
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
TP53, P53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53 |
Lieferbar |
|
Primary Accession |
P04637 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting. |
Bio References |
Chen L, et al. Oncogene. 2003 Aug 14; 22(34):5348-57.Melchior F, et al. Cell Cycle. 2002 Jul-Aug; 1(4):245-9. Kahyo T, et al. Mol Cell. 2001 Sep; 8(3):713-8. Kwek SS, et al. Oncogene. 2001 May 3; 20(20):2587-99. Muller S, et al. J Biol Chem. 2000 May 5; 275(18):13321-9. Gostissa M, et al. EMBO J. 1999 Nov 15; 18(22):6462-71. Rodriguez MS, et al. EMBO J. 1999 Nov 15; 18(22):6455-61. |
Clonality |
Polyclonal |
Clone/Animal Names |
RB2571 |
Gene ID |
7157 |
Gene Name |
TP53 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
TP53 Antibody (C-term) (Cat. #AP2505b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TP53 antibody detected the TP53 protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
All lanes : Anti-Sumo-site Antibody (p53) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HT-29 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Legend image 3 |
Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific)(Cat#AP2505b). AP2505b was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:100 |
Isotype |
Rabbit Ig |
Calculated MW |
43653 |
Antigen Region |
364-393 |
Antigen Source |
HUMAN |
Target/Specificity |
This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53. |
NCBI Accession |
NP_000537.3; NP_001119584.1; NP_001119585.1; NP_001119586.1; NP_001119587.1; NP_001119588.1; NP_001119589.1; NP_001119590.1; NP_001263624.1; NP_001263625.1; NP_001263626.1; NP_001263627.1; NP_001263628.1; NP_00126 |
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