ArtNr |
ABC-AP22298a |
Hersteller |
Abcepta
|
Menge |
200 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
TP53, P53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53 |
Lieferbar |
|
Primary Accession |
P04637 |
Antigen Type |
Synthesized Peptide |
Application |
WB |
Bio Background |
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. |
Bio References |
Zakut-Houri R., et al.EMBO J. 4:1251-1255(1985).Lamb P., et al.Mol. Cell. Biol. 6:1379-1385(1986).Harlow E., et al.Mol. Cell. Biol. 5:1601-1610(1985).Harris N., et al.Mol. Cell. Biol. 6:4650-4656(1986).Buchman V.L., et al.Gene 70:245-252(1988). |
Clonality |
polyclonal |
Clone/Animal Names |
RB57840 |
Gene ID |
7157 |
Gene Name |
TP53 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Anti- p53 (S376) Antibody at 1:2000 dilution + HT-29 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
All lanes : Anti-p53(S376) Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HT-29 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Isotype |
Rabbit Ig |
Calculated MW |
43653 |
Antigen Source |
HUMAN |
Target/Specificity |
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 349-385 amino acids from human. |
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