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FADS2 Antibody

FADS2 Antibody

ArtNr	ABC-AP22275a
Hersteller	Abcepta
Menge	200 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	FADS2, Fatty acid desaturase 2, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, 1.14.19.-
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Primary Accession	O95864
Antigen Type	Recombinant Protein
Application	WB, IF
Bio Background	Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
Bio References	Cho H.P., et al.J. Biol. Chem. 274:471-477(1999). Marquardt A., et al.Genomics 66:175-183(2000). Zhang J.S.S., et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases. Ota T., et al.Nat. Genet. 36:40-45(2004). Otsuki T., et al.DNA Res. 12:117-126(2005).
Clonality	polyclonal
Clone/Animal Names	RB56816
Gene ID	9415
Gene Name	FADS2 (HGNC:3575)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling FADS2 with AP22275a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	Anti-FADS2 Antibody at 1:2000 dilution + Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Isotype	Rabbit Ig
Calculated MW	52259
Antigen Source	HUMAN
Target/Specificity	This FADS2 antibody is generated from a rabbit immunized with a recombinant protein of human FADS2.

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ABC-AP22275a-datasheet.pdf

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Menge: 200 ul

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