SLC2A8 Antibody (Center)

Synthesized Peptide

Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose (By similarity).

polyclonal

29988

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-SLC2A8 Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: human testis lysate
Lane 4: Caco2 whole cell lysate
Lane 5: mouse testis lysate
Lane 6: rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 51 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P

Overlay histogram showing U-2 OS cells stained with AP22138c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22138c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

B

50819

This SLC2A8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 260-292 amino acids from the Central region of human SLC2A8.