SLC29A2 Antibody (N-Term)

Q14542

WB, IHC-P, FC

Williams J.B., et al.Biochem. Biophys. Res. Commun. 213:325-333(1995).
Griffiths M., et al.Biochem. J. 328:739-743(1997).
Crawford C.R., et al.J. Biol. Chem. 273:5288-5293(1998).
Mangravite L.M., et al.Am. J. Physiol. 284:F902-F910(2003).
Ota T., et al.Nat. Genet. 36:40-45(2004).

RB55890

SLC29A2

H

WB

AP22096a staining SLC29A2 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

1:25

IHC-P

Overlay histogram showing HepG2 cells stained with AP22096a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22096a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

50113

This SLC29A2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-31 amino acids from human SLC29A2.