IRF3 Antibody (N-Term)

Q14653

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

polyclonal

3661

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-IRF3 Antibody (N-Term) at 1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 47 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

47219

This IRF3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from human IRF3.