COPS5 Antibody (Center)

Q92905

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.

polyclonal

10987

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-COPS5 Antibody (Center) at 1:500-1:1000 dilution
Lane 1: NIH/3T3 whole cell lysates
Lane 2: U-87 MG whole cell lysates
Lane 3: PC-12 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 38 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:500-1:1000

37579

This COPS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 187-222 amino acids from the Central region of human COPS5.