ArtNr |
ABC-AP2140b-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
USP12, UBH1, USP12L1, Ubiquitin carboxyl-terminal hydrolase 12, Deubiquitinating enzyme 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12 |
Lieferbar |
|
Primary Accession |
O75317 |
Antigen Type |
Synthetic Peptide |
Application |
WB |
Bio Background |
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs), 1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions. |
Bio References |
Hansen-Hagge, T.E., et al., Genomics 49(3):411-418 (1998). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB4300 |
Gene ID |
219333 |
Gene Name |
USP12 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of USP12/USP46 Antibody (C-term) (Cat. #AP2140b) in MDA-MB231 cell line lysates (35ug/lane). USP12/USP46 (arrow) was detected using the purified Pab. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of USP12 (arrow) using rabbit polyclonal USP12 Antibody (L315) (Cat. #AP2140b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP12 gene. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Other Accession |
P62069; P62068; A5WWB0; Q9D9M2; A4FUN7; A5D9H7; Q5M981; Q52KZ6; NP_872294; F1M625 |
Predicted |
X, B, Zf, M, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
42858 |
Antigen Region |
315-345 |
Antigen Source |
HUMAN |
Target/Specificity |
This USP12/USP46 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-345 amino acids from the C-terminal region of human USP12/USP46. |
NCBI Accession |
NP_872294.2 |
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