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DHFR Antibody (N-term)

DHFR Antibody (N-term)

ArtNr	ABC-AP20626a-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	DHFR, Dihydrofolate reductase
Lieferbar
Primary Accession	P00374
Application	IHC-P, WB
Bio Background	Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Bio References	Chen M.-J., et al.J. Biol. Chem. 259:3933-3943(1984). Masters J.N., et al.Gene 21:59-63(1983). Yang J.K., et al.J. Mol. Biol. 176:169-187(1984). Schmutz J., et al.Nature 431:268-274(2004). Banka S., et al.Am. J. Hum. Genet. 88:216-225(2011).
Clonality	Polyclonal
Clone/Animal Names	RB49346
Gene ID	1719
Gene Name	DHFR
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	Western blot analysis of lysates from 293T, Hela, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate(from left to right), using DHFR Antibody (N-term)(Cat. #AP20626a). AP20626a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Immunohistochemical analysis of paraffin-embedded H. liver section using DHFR Antibody (N-term)(Cat#AP20626a). AP20626a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Type image 2	IHC-P
Dilution image 2	1:25
Legend image 3	Immunohistochemical analysis of paraffin-embedded H. brain section using DHFR Antibody (N-term)(Cat#AP20626a). AP20626a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Type image 3	IHC-P
Dilution image 3	1:25
Other Accession	P00377; P00375; P00376
Predicted	B, Pig
Isotype	Rabbit Ig
Calculated MW	21453
Antigen Source	HUMAN
Target/Specificity	This DHFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human DHFR.

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ABC-AP20626a-ev-datasheet.pdf

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Menge: 80 ul

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