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HLA-B Antibody (N-term)

HLA-B Antibody (N-term)

ArtNr	ABC-AP20563a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HLA-B, HLA class I histocompatibility antigen, HLAB, B-15 alpha chain, MHC class I antigen B*15
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Primary Accession	P30464
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	Involved in the presentation of foreign antigens to the immune system.
Bio References	Little A.-M., et al.Tissue Antigens 38:186-190(1991). Hildebrand W.H., et al.Tissue Antigens 43:209-218(1994). Lin L., et al.Tissue Antigens 47:265-274(1996). Madrigal J.A., et al.J. Immunol. 149:3411-3415(1992). Watkins D.I., et al.Nature 357:329-333(1992).
Clonality	Polyclonal
Clone/Animal Names	RB47637
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-B Antibody (N-term)(Cat#AP20563a). AP20563a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 3	IHC-P
Dilution image 3	1:25
Legend image 4	Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-B Antibody (N-term)(Cat#AP20563a). AP20563a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 4	IHC-P
Dilution image 4	1:25
Legend image 5	Immunohistochemical analysis of paraffin-embedded R. spleen section using HLA-B Antibody (N-term)(Cat#AP20563a). AP20563a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 5	IHC-P
Dilution image 5	1:25
Legend image 6	AP20563a staining HLA-B in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 6	IHC-P
Dilution image 6	1:25
Other Accession	P30488; P30486; P30483; P30479; Q04826; P30685; P30466
Isotype	Rabbit Ig
Antigen Source	HUMAN
Target/Specificity	This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 75-107 amino acids from the N-terminal region of human HLA-B.

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ABC-AP20563a-datasheet.pdf

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Menge: 400 ul

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Lieferung vsl. bis 04.10.2024

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