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POLR2C Antibody (C-term)

POLR2C Antibody (C-term)

ArtNr	ABC-AP20520b
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	POLR2C, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB33, RPB31, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C
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Primary Accession	P19387
Antigen Type	Synthetic Peptide
Application	IF, WB, IHC-P
Bio Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Bio References	Pati U.K., et al. J. Biol. Chem. 265:8400-8403(1990). Dammann R., et al. Biochim. Biophys. Acta 1396:153-157(1998). Bruno T., et al. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases. Loftus B.J., et al. Genomics 60:295-308(1999). Bienvenut W.V., et al. Submitted (MAR-2009) to UniProtKB.
Clonality	Polyclonal
Gene ID	5432
Gene Name	POLR2C (HGNC:9189)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Fluorescent image of Hela cells stained with POLR2C Antibody (C-term)(Cat#AP20520B). AP20520B was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	POLR2C Antibody (C-term) (Cat. #AP20520b) western blot analysis in Hela, U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded H.brain, H.cerebellum tissue reacted with POLR2C Antibody (C-term) (Cat#AP20520b).
Type image 3	IHC-P
Dilution image 3	1:100
Other Accession	P97760; Q3T0Q3
Predicted	B
Isotype	Rabbit Ig
Calculated MW	31441
Antigen Region	200-228
Antigen Source	Human
Target/Specificity	This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C.
NCBI Accession	NP_116558.1

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ABC-AP20520b-datasheet.pdf

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Menge: 400 ul

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