KDELR Antibody (C-term)

Synthetic Peptide

Retention of resident soluble proteins in the lumen of the
endoplasmic reticulum (ER) is achieved in both yeast and animal
cells by their continual retrieval from the cis-Golgi, or a
pre-Golgi compartment. Sorting of these proteins is dependent on a
C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in
animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This
process is mediated by a receptor that recognizes, and binds the
tetrapeptide-containing protein, and returns it to the ER. In
yeast, the sorting receptor encoded by a single gene, ERD2, which
is a seven-transmembrane protein. Unlike yeast, several human
homologs of the ERD2 gene, constituting the KDEL receptor gene
family, have been described. The protein encoded by this gene was
the first member of the family to be identified, and it encodes a
protein structurally and functionally similar to the yeast ERD2
gene product.

Polyclonal

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-KDELR1 Antibody (C-term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: 293 whole cell lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 25 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

X, B, M, Rat, C, Zf

185-211

This KDELR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the C-terminal region of human KDELR1.