ArtNr |
ABC-AP1813C-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7, APG7-like |
Lieferbar |
|
Primary Accession |
O95352 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy. |
Bio References |
References for protein:1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)3. Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4.Levine B. Cell. 120(2):159-62. (2005) 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)6. Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002) 7.Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002)References for U251 cell line:1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J., Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].3. Geng Y.; Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898]. |
Clonality |
Polyclonal |
Clone/Animal Names |
RB7472 |
Gene ID |
10533 |
Gene Name |
ATG7 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
All lanes : Anti-APG7L Antibody (R509) at 1:1000 dilutionLane 1: Ba/F3 whole cell lysateLane 2: Caki-1 whole cell lysateLane 3: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
AP1813C staining APG7L in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:25 |
Other Accession |
Q641Y5; Q9D906 |
Predicted |
Rat |
Isotype |
Rabbit Ig |
Calculated MW |
77960 |
Antigen Region |
494-523 |
Antigen Source |
HUMAN |
Target/Specificity |
This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7. |
NCBI Accession |
NP_001129503.2; NP_001138384.1; NP_006386.1 |
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