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UBE4A Antibody (Center)

UBE4A Antibody (Center)

ArtNr	ABC-AP14678c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 5.1	32160702
ECLASS 6.1	32160702
ECLASS 8.0	32160702
ECLASS 9.0	32160702
ECLASS 10.0.1	32160702
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499)
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Primary Accession	Q14139
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.
Bio References	Shi, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16275-16280(2009) Sakiyama, T., et al. Inflamm. Bowel Dis. 14(3):310-317(2008) Yang, Y., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23(6):504-506(2007) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : Spinette, S., et al. Cell Cycle 3(12):1638-1644(2004)
Clonality	Polyclonal
Clone/Animal Names	RB34528
Gene ID	9354
Gene Name	UBE4A (HGNC:12499)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	UBE4A Antibody (Center) (Cat. #AP14678c) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the UBE4A antibody detected the UBE4A protein (arrow).
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	All lanes : Anti-UBE4A Antibody (Center) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	UBE4A Antibody (Center) (AP14678c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Other Accession	A5PKG6; NP_004779.2
Predicted	B
Isotype	Rabbit Ig
Calculated MW	122561
Antigen Region	451-479
Antigen Source	HUMAN
Target/Specificity	This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human UBE4A.
NCBI Accession	NP_001191006.1; NP_004779.2

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ABC-AP14678c-datasheet.pdf

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Menge: 400 ul

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