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DPPA4 Antibody (N-term)

DPPA4 Antibody (N-term)

ArtNr	ABC-AP1438a-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	DPPA4, Developmental pluripotency-associated protein 4
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Primary Accession	Q7L190
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	DppA4 is a member of the Dppa family of developmental pluripotency associated transcription factors. Dppa4 is a target of Oct4, Sox2 and Nanog. It may play a role in maintaining cell pluripotentiality.
Bio References	Bortvin, A., Development 130 (8), 1673-1680 (2003
Clonality	Polyclonal
Clone/Animal Names	RB13363
Gene ID	55211
Gene Name	DPPA4
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-DPPA4 Antibody (N-term) at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of DPPA4 Antibody (N-term) (Cat. #AP1438a) in CEM cell line lysates (35ug/lane). DPPA4 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	AP1438a staining DPPA4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:25
Isotype	Rabbit Ig
Calculated MW	33541
Antigen Region	50-79
Antigen Source	HUMAN
Target/Specificity	This DPPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human DPPA4.
NCBI Accession	NP_060659.3

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ABC-AP1438a-ev-datasheet.pdf

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Menge: 80 ul

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