ArtNr |
ABC-AP13697c |
Hersteller |
Abcepta
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CRYAB, CRYA2, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5 |
Lieferbar |
|
Primary Accession |
P02511 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Crystallins are separated into two classes:taxon-specific, or enzyme, and ubiquitous. The latter classconstitutes the major proteins of vertebrate eye lens and maintainsthe transparency and refractive index of the lens. Since lenscentral fiber cells lose their nuclei during development, thesecrystallins are made and then retained throughout life, making themextremely stable proteins. Mammalian lens crystallins are dividedinto alpha, beta, and gamma families; beta and gamma crystallinsare also considered as a superfamily. Alpha and beta families arefurther divided into acidic and basic groups. Seven protein regionsexist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed oftwo gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock andare members of the small heat shock protein (sHSP also known as theHSP20) family. They act as molecular chaperones although they donot renature proteins and release them in the fashion of a truechaperone; instead they hold them in large soluble aggregates.Post-translational modifications decrease the ability to chaperone.These heterogeneous aggregates consist of 30-40 subunits; thealpha-A and alpha-B subunits have a 3:1 ratio, respectively. Twoadditional functions of alpha crystallins are an autokinaseactivity and participation in the intracellular architecture.Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B isexpressed widely in many tissues and organs. Elevated expression ofalpha-B crystallin occurs in many neurological diseases; a missensemutation cosegregated in a family with a desmin-related myopathy. |
Bio References |
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)Jehle, S., et al. Nat. Struct. Mol. Biol. 17(9):1037-1042(2010)Kida, E., et al. J. Neuropathol. Exp. Neurol. 69(7):745-759(2010)Deng, Y., et al. BMB Rep 43(6):432-437(2010)Houck, S.A., et al. PLoS ONE 5 (7), E11795 (2010) : |
Clonality |
Polyclonal |
Clone/Animal Names |
RB33613 |
Gene ID |
1410 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Western blot analysis of CRYAB (arrow) using rabbit polyclonal CRYAB Antibody (Center) (Cat. #AP13697c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRYAB gene. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
CRYAB Antibody (Center) (Cat. #AP13697c) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the CRYAB antibody detected the CRYAB protein (arrow). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
CRYA Antibody (Center) (Cat. #AP13697c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Other Accession |
P23928; P41316; Q7M2W6; P23927; Q60HG8; P02510; NP_001876.1; Q5ENY9 |
Predicted |
B, Pr, Pig, Rb, Rat, Sh |
Isotype |
Rabbit Ig |
Calculated MW |
20159 |
Antigen Region |
84-112 |
Antigen Source |
HUMAN |
Target/Specificity |
This CRYAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the Central region of human CRYAB. |
NCBI Accession |
NP_001876.1 |
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