CRYAB Antibody (Center)

Synthetic Peptide

Crystallins are separated into two classes:
taxon-specific, or enzyme, and ubiquitous. The latter class
constitutes the major proteins of vertebrate eye lens and maintains
the transparency and refractive index of the lens. Since lens
central fiber cells lose their nuclei during development, these
crystallins are made and then retained throughout life, making them
extremely stable proteins. Mammalian lens crystallins are divided
into alpha, beta, and gamma families; beta and gamma crystallins
are also considered as a superfamily. Alpha and beta families are
further divided into acidic and basic groups. Seven protein regions
exist in crystallins: four homologous motifs, a connecting peptide,
and N- and C-terminal extensions. Alpha crystallins are composed of
two gene products: alpha-A and alpha-B, for acidic and basic,
respectively. Alpha crystallins can be induced by heat shock and
are members of the small heat shock protein (sHSP also known as the
HSP20) family. They act as molecular chaperones although they do
not renature proteins and release them in the fashion of a true
chaperone; instead they hold them in large soluble aggregates.
Post-translational modifications decrease the ability to chaperone.
These heterogeneous aggregates consist of 30-40 subunits; the
alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two
additional functions of alpha crystallins are an autokinase
activity and participation in the intracellular architecture.
Alpha-A and alpha-B gene products are differentially expressed;
alpha-A is preferentially restricted to the lens and alpha-B is
expressed widely in many tissues and organs. Elevated expression of
alpha-B crystallin occurs in many neurological diseases; a missense
mutation cosegregated in a family with a desmin-related myopathy.

Polyclonal

1410

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of CRYAB (arrow) using rabbit polyclonal CRYAB Antibody (Center) (Cat. #AP13697c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRYAB gene.

1:1000

WB

CRYA Antibody (Center) (Cat. #AP13697c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

1:10~50

B, Pr, Pig, Rb, Rat, Sh

20159

HUMAN

NP_001876.1