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Puma BH3 Domain Antibody

Puma BH3 Domain Antibody

ArtNr	ABC-AP1317a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PUMA, BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
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Primary Accession	Q9BXH1
Antigen Type	Synthetic Peptide
Application	FC, IF, IHC-P, WB
Bio Background	PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Bio References	Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Hoque, M.O., et al., Cancer Lett. 199(1):75-81 (2003). Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(4):1931-1936 (2003). Han, J., et al., Proc. Natl. Acad. Sci. U.S.A. 98(20):11318-11323 (2001). Nakano, K., et al., Mol. Cell 7(3):683-694 (2001).
Clonality	Polyclonal
Clone/Animal Names	RB01353
Gene ID	27113
Gene Name	BBC3
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of Puma BH3 Domain Antibody(Cat#AP1317a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of anti-Puma BH3 domain Pab (Cat. #AP1317a) in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (Cat.#AP1317a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Puma BH3 Domain Antibody (Cat. #AP1317a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	Q80ZG6; Q99ML1
Predicted	M, Rat
Isotype	Rabbit Ig
Calculated MW	20532
Antigen Region	119-154
Antigen Source	HUMAN
Target/Specificity	This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.
NCBI Accession	NP_001120712.1; NP_001120713.1; NP_001120714.1; NP_055232.1

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ABC-AP1317a-datasheet.pdf

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Menge: 400 ul

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