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COL8A2 Antibody (C-term)

COL8A2 Antibody (C-term)

ArtNr	ABC-AP11859B-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	COL8A2, Collagen alpha-2(VIII) chain, Endothelial collagen
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Primary Accession	P25067
Antigen Type	Synthetic Peptide
Application	IHC-P-Leica, IF, WB
Bio Background	This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.
Bio References	Hemadevi, B., et al. BMC Ophthalmol 10, 3 (2010) : Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009) Liskova, P., et al. Br J Ophthalmol 91(12):1717-1718(2007) Aldave, A.J., et al. Cornea 26(8):963-965(2007) Zhang, C., et al. Trans Am Ophthalmol Soc 104, 85-97 (2006) :
Clonality	Polyclonal
Clone/Animal Names	RB31742
Gene ID	1296
Gene Name	COL8A2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Confocal immunofluorescent analysis of COL8A2 Antibody (C-term)(Cat#AP11859b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	All lanes : Anti-COL8A2 Antibody (C-term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Neuro-2a whole cell lysate Lane 4: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Immunohistochemical analysis of paraffin-embedded Human testis tissue using AP11859B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P-Leica
Dilution image 3	1:500
Legend image 4	Immunohistochemical analysis of paraffin-embedded Human kidney tissue using AP11859B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 4	IHC-P-Leica
Dilution image 4	1:500
Other Accession	NP_005193.1
Isotype	Rabbit Ig
Calculated MW	67244
Antigen Region	571-599
Antigen Source	HUMAN
Target/Specificity	This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2.
NCBI Accession	NP_005193.1

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ABC-AP11859B-ev-datasheet.pdf

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Menge: 80 ul

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