HDAC2 Antibody (C-term)

Q92769

WB, IF, IHC-P

Choi, Y.B., et al., J. Biol. Chem. 279(49):50930-50941 (2004).
Zhu, P., et al., Cancer Cell 5(5):455-463 (2004).
Longworth, M.S., et al., J. Virol. 78(7):3533-3541 (2004).
Lu, Y., et al., J. Biol. Chem. 278(48):47792-47802 (2003).
Verdin, E., et al., Trends Genet. 19(5):286-293 (2003).

RB5718

HDAC2 (HGNC:4853)

H

IF

Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term)(Cat#AP1102b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.

1:10~50

WB

Western blot analysis of anti-HDAC2 Antibody (C-term) (Cat.#AP1102b) in 293 cell line lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.

1:1000

WB

HDAC2 Antibody (C-term) (Cat. #AP1102b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

1:10~50

55364

HUMAN

NP_001518.3