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PEMT Antibody (C-term)

PEMT Antibody (C-term)

ArtNr	ABC-AP1025B-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PNMT, PEMT, PEMPT, Phosphatidylethanolamine N-methyltransferase, PEMT2, PEAMT
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Primary Accession	Q9UBM1
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17.
Bio References	Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999). Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001). Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Clonality	Polyclonal
Clone/Animal Names	RB12628
Gene ID	10400
Gene Name	PEMT {ECO:0000255\|HAMAP-Rule:MF_03216}
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-PEMT Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse ovary tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Immunohistochemical analysis of AP1025B on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:100
Legend image 3	Immunohistochemical analysis of AP1025B on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:100
Isotype	Rabbit Ig
Calculated MW	22134
Antigen Region	170-199
Antigen Source	HUMAN
Target/Specificity	This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PEMT.
NCBI Accession	NP_001254480.1; NP_001254481.1; NP_009100.2; NP_680477.1; NP_680478.1

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ABC-AP1025B-ev-datasheet.pdf

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Menge: 80 ul

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