ArtNr |
ABC-AP1025B-ev |
Hersteller |
Abcepta
|
Menge |
80 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
PNMT, PEMT, PEMPT, Phosphatidylethanolamine N-methyltransferase, PEMT2, PEAMT |
Lieferbar |
|
Primary Accession |
Q9UBM1 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. |
Bio References |
Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999).Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001).Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB12628 |
Gene ID |
10400 |
Gene Name |
PEMT {ECO:0000255|HAMAP-Rule:MF_03216} |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
All lanes : Anti-PEMT Antibody (C-term) at 1:1000 dilutionLane 1: A549 whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: Mouse liver tissue lysateLane 4: Mouse ovary tissue lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Immunohistochemical analysis of AP1025B on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:100 |
Legend image 3 |
Immunohistochemical analysis of AP1025B on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:100 |
Isotype |
Rabbit Ig |
Calculated MW |
22134 |
Antigen Region |
170-199 |
Antigen Source |
HUMAN |
Target/Specificity |
This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PEMT. |
NCBI Accession |
NP_001254480.1; NP_001254481.1; NP_009100.2; NP_680477.1; NP_680478.1 |
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