MRPL42 Antibody

Q9Y6G3

1. Genomics. 2003 May; 81(5):468-80. 2. J Biol Chem. 2001 Nov 23; 276(47):43958-69.

28977

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);

1/10000

WB

Figure 2: Western blot analysis using MRPL42 mAb against HEK293 (1) and MRPL42 (AA: 10-142)-hIgGFc transfected HEK293 (2) cell lysate.

1/500 - 1/2000

WB

Figure 4: Flow cytometric analysis of HepG2 cells using MRPL42 mouse mAb (green) and negative control (purple).

1/200 - 1/400

IHC

Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MRPL42 mouse mAb with DAB staining.

1/200 - 1/1000

16661

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.

Purified recombinant fragment of human MRPL42 (AA: 10-142) expressed in E. Coli.