ArtNr |
ABC-AO2065a |
Hersteller |
Abcepta
|
Menge |
100 ul |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, FC, ICC, ELISA |
Clon |
2D9E8 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
1-phosphatidylinositol 4, PLCG2, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phospholipase C-gamma-2, Phospholipase C-IV, Phosphoinositide phospholipase C-gamma-2, 3.1.4.11 |
Lieferbar |
|
Primary Accession |
P16885 |
Application |
WB, FC, ICC, E |
Bio References |
1.J Biol Chem. 2005 Nov 25; 280(47):38923-31.2.World J Gastroenterol. 2003 Nov; 9(11):2413-8. |
Clonality |
Monoclonal |
Gene ID |
5336 |
Gene Name |
|
Subtitle |
Purified Mouse Monoclonal Antibody |
Reactivity |
H |
Legend image 1 |
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); |
Type image 1 |
E |
Dilution image 1 |
1/10000 |
Legend image 2 |
Figure 2:Western blot analysis using PLCG2 mAb against human PLCG2 (AA: 826-985) recombinant protein. (Expected MW is 44.6 kDa) |
Type image 2 |
WB |
Dilution image 2 |
1/500 - 1/2000 |
Legend image 3 |
Figure 3:Western blot analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate. |
Type image 3 |
WB |
Dilution image 3 |
1/500 - 1/2000 |
Legend image 4 |
Figure 4:Immunofluorescence analysis of MCF-7 cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
Type image 4 |
IF |
Dilution image 4 |
1/200 - 1/1000 |
Legend image 5 |
Figure 5:Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red). |
Type image 5 |
FC |
Dilution image 5 |
1/200 - 1/400 |
Isotype |
IgG1 |
Calculated MW |
147870 |
Formulation |
Purified antibody in PBS with 0.05% sodium azide |
Description |
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. |
|
Clone: 2D9E8 |
Immunogen |
Purified recombinant fragment of human PLCG2 (AA: 826-985) expressed in E. Coli. |
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